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The chromatographic separation was achieved using an Agilent Eclipse XDB C 8 (150 × 4.6 mm, 5 μ ) column, the optimized method used a segmented gradient mobile phase with phosphate buffer at pH = 2.5 as solvent (A) and ACN as solvent (B), and the gradient program is shown in Table 1

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L-leucine increases the sensitivity of drug-resistant Salmonella to sarafloxacin by stimulating central carbon metabolism and increasing intracellular reactive oxygen species level