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Given the potential impact of these factors on the quantification of GSH, here we examined if the type of anticoagulant used during sample collection (heparin or EDTA), deproteinization of plasma prior to storage (yes or no), storage temperature (−80 o C or 4 o C), and processing time (duration of time between collection of whole blood and deproteinization of plasma) impact levels of free GSH and GSSG, in plasma