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Briefly, M0 macrophages were stimulated with lipopolysaccharide (LPS) and interferon γ (IFN-γ) to switch to the M1 phenotype as a positive control, while those stimulated with interleukin-4 (IL-4) to switch to the M2 phenotype served as a negative control

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Interestingly, suppressing the ER stress pathway PERK-ATF4-CHOP did not affect CHAC1 expression during ferroptosis ( As controlled induction of ferroptosis might prove a promising target for cancer research, various pharmacological substances have been tested for possibly inducing ferroptosis