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In adipocyte studies, 5-amino-1MQ treatment produced measurable increases in intracellular NAD+ without any NAD+ or precursor being administered, which is the mechanistic basis researchers cite when describing it as NAD+-supportive rather than NAD+-replenishing

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The TB-500 component — thymosin β-4 — is prohibited at all times under section S2 of the WADA Prohibited List (peptide hormones, growth factors, related substances and mimetics), which expressly names thymosin-β-4 and its derivatives