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Vesicular GABA Transporter/Vesicular Inhibitory Amino Acid Transporter Once synthesized, GABA is packaged into synaptic vesicles (SVs) which are then trafficked to the presynaptic membrane to fuse with and to release the neurotransmitter to the synaptic cleft in response to the increase in intracellular Ca 2+ concentration (Figure 3) [76,77]