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Pull down assay for GPX4 PC12 cells were treated with DAQ probe (DA-yne probe contains FeCl 3 solution) or DMSO for 6 hours, and then were harvested and lysed with RIPA buffer supplemented with protease inhibitor cocktails and phosphatase inhibitor cocktails

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In clinical practice, patients will receive a prescription for ipamorelin with dosage guidelines, which likely vary from clinic to clinic and physician to physician