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As a positive control for apoptosis, tamoxifen (50 μM) was included ( 2 , 1.2 mM EGTA, 1.5% Nonidet P40, 0.3% CHAPS, 30% sucrose, 150 μM caspase 3 substrate DEVD-AMC, 30 mM DTT, and 3.0 mM PMSF was directly added to HepG2 cells

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Besides insulin, GPI-PLD expression is associated with lipid levels [95,96,97] and its activity is also associated with triglyceride [98] and lipid metabolism in the liver [99], which may be somehow related to the improved lipid profiles of patients suffering from metabolic diseases after supplementation with inositols like DCI [100]